Obituary: Klaus Biemann.

Shotgun Protein Sequencing: Assembly of Tandem Mass Spectra from Mixtures of Modified Proteins

Despite significant advances in the identification of known proteins, the analysis of unknown proteins by tandem mass spectrometry (MS/MS) still remains a challenging open problem. Although Klaus Biemann recognized the potential of tandem mass spectrometry (MS/MS) for sequencing of unknown proteins in the 1980s, low-throughput Edman degradation followed by cloning still remains the main method ...

Obituary

Obituary: Professor Parvaneh Vossough

Structure Determination of Glycoproteins by Mass Spectrometry

The relevant background information about the glycoprotein, enzymatic digestion, and the new matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDITOF MS) ionization technique is reviewed. This method allows the detection of a complex mixture over a wide mass range (500-200,000 Da) using picomole to femtomole amounts of sample. Therefore, it is now possible to deter...

Fast atom bombardment-mass spectrometry strategies for analysing glycoprotein glycans.

14. Barber, M., Bordoli, R. S., Sedgwick, R. D. & ‘lylcr, A. N . ( 198 I ) J. Chem. Soc. Chem. Commirri. 7,325-327 Biemann, K. & Martin, S. A. ( 1 987) Moss Spectrom. Rev. 6 , 1-76 Gibson, B. W. & Biemann, K. (1984) /’roc. Nor/. Aciid. Sci. U.S.A. 8 I , 1956I960 Allmaier, G., Chao, B. H., Khorana, H. G. & Biemann, K. ( 1986) in Annual Conference on Moss Spectrometty w i r l Allied Topics 34h, p...